On May 17, the team of Han Bin, a researcher at the Center for Excellence in Molecular Plants of the Chinese Academy of Sciences, and the team of Shanghai Zhongke Quanyin Molecular Breeding Technology Co., Ltd., Anhui Quanyin High-tech Seed Industry Co., Ltd., and the Institute of Crop Science and Bioinformatics of Zhejiang University published their latest research papers in the international journal Rice Science. Through the close cooperation between the rice genome research team and the high-level breeding team, this study analyzes the breeding and matching process of the trilineage sterile line of rice with the largest application area in China – Quan 9311A for the first time in the world from the genome level, and analyzes the logic of the aggregation of dominant traits of breeders at the genetic level, especially how to pioneer the use of two-line recovery line materials, which provides a new path and genomic support method for the creation of excellent sterile lines.
Located in Nancheng County, Jiangxi Province, the Tsuen 9311A Planting Field Photo courtesy of Fang Yu, the same below
This work allows everyone to better match and improve the dominant parent and backbone germplasm Tsuen 9311A through the method of “retrospective”; It can also enable those engaged in basic research to understand breeding, so as to develop better molecular biology tools for breeding and improve breeding efficiency. From the perspectives of breeders and genomics researchers, this study builds a bridge between the deep integration of basic research and breeding practice to build a breeding system for rice genome design.
The research team learned in detail about the breeding process of Quan 9311A: the parents of Quan 9311A are 93-11 and middle 9A, the father of the matching group is Wushan silk seedlings, and the F1 generation seed is Quanyou silk seedlings (the promotion area in 2022 will reach 2.53 million mu). The team performed deep sequencing of the above materials and performed a “physical examination” analysis of the genome sequencing results, summarizing and comparing the most important genes associated with key agronomic traits in relevant breeding materials and varieties.
Located in Nancheng County, Jiangxi Province, Tsuen 9311A farming
Studies suggest that traits and genotypes still need to promote each other during breeding. Field traits that breeders pay special attention to have no genotype support, such as strong coordination. Genetic analysis has revealed many excellent genes, whose commercial value still needs to be tapped: such as salt tolerance genes.
Secondly, breeding urgently needs technical means such as genomics to improve efficiency. The successful selection of Tsuen 9311A depends not only on the ingenious ideas of the breeders, but also on the great hardship and endurance of the breeders in the process of screening each generation of huge groups, which requires advanced technologies including genomics to support, thereby reducing the workload and speeding up breeding.
Thirdly, the development of genomic technology still faces some problems, such as molecular theory research often only evaluates the traits of a single gene and a single site, which cannot truly reflect the breeding value of the relevant site. Some genes play a role in epistatic interactions, forming a regulatory network to produce target traits, and the evaluation of only one site often does not reflect the actual effect in the breeding combination. The genomic breeding tools currently developed are not well developed and are not easy to understand.
Li Qianlong of Shanghai Zhongke Quanyin Molecular Breeding Technology Co., Ltd. is the first author of this paper, Wang Ahong, associate researcher of the Center of Excellence of Molecular Plants of the Chinese Academy of Sciences, and Fang Yu, researcher of Shanghai Zhongke Quanyin Molecular Breeding Technology Co., Ltd. and the Institute of Crop Science and Bioinformatics of Zhejiang University, are co-corresponding authors.
The research was supported by the National Natural Science Foundation of China, the Shanghai Science and Technology Rejuvenation Project, and the National Rice Industry Technology System. (Source: Li Chen, China Science News)
Related paper information:https://doi.org/10.21203/rs.3.rs-1727782/v1
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